I would like to do a study on a variety of knock-in knock-out mice (both at baseline and high fat diet). What would be a good age to sacrifice the mice? How long should our mice be kept on a high fat diet?
Ideally I want to see a change in cholesterol levels. - Particularly LDL levels.
Yes, after three weeks from starting experiment, this will give good results in hematology of mice (scarified)
Mier Kestecher There is no straightforward answer to your question since mice can be sacrificed at various age periods depending on the study designs or disease models being developed or investigated. Here, you are looking at the effect of high fat diet on some knock in and knock out genes in mice. It may take a while for dyslipidemia to develop in rodents following high fat diet feeding. This is dependent on a number of factors such as the age of the mice at exposure to high fat diet and also the type of high fat diet administered. You need to do a literature search on those genes to determine if a similar study exists. This will guide you.
