How can I neglect heat of dilution from heat of reaction in Isothermal Titration Calorimetry?

In my ITC run (Nano ITC, TA instruments), the heat of reaction is less than heat of dilution. For e.g. the heat of reaction for 1st and 2nd titrations was 4.17uJ and 6.23uJ respectively whereas...

02 March 2015 1,995 8 View

Why purified protein band shifted to lower molecular weight in SDS-PAGE?

I am trying to purify His-tagged protein (by Ni-NTA resin) after over-expression in BL21 cells. When i run SDS-PAGE of un-induced, induced, Lysate, supernatant, pellet and purified protein...

09 October 2014 4,913 12 View

Why am I seeing secondary structural changes in molecular dynamic simulation?

There are missing residues in the crystal structure of my protein. One part of this missing residue region (30 residues length) has no template. When I modelled this protein in modeller by taking...

04 May 2014 3,735 3 View

Temperature for the annealing step in PCR?

I am having one gene whose melting temperature for forward primer is 63 and for reverse its 70. Up to what temperature should I run the annealing temperature? Until now I did a gradient of 50-60...

02 March 2013 3,158 5 View

What are the general principles of isolating particular protein from proteome?

I have isolated proteome of a bacteria and I want to isolate and purify particular proteins from them. I am not able to understand how to design a protocol for isolation and purification of them....

02 March 2013 8,645 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View