The usual time for fasting plasma sugar is 8-10 hours, the test may be invalid before or after due to the hepatic and meal glucose. No fasting is needed for the total cholesterol estimation. The time needed for the triglycerides is 12 hours, the LDL can be estimated by a formula in case of TG not 400 mg or more.
In clinics, patients are called after an overnight fasting period, which is known to be around 10 hours. The sample will be valid for the assay of all standard biochemical parameters including glucose and lipids.
I think 10 hours is a better bet but you will have to statardize your protocol and also the time of estimation. For lipid profile I think 12 hours are better but again your experment protocolial is important in this connection. previous dietary history, if known, can give you a clue for a better time to decide.
8-10 hours is the time period for fasting for both glucose and lipids. Too long a fasting can induce hypoxia and cause hydrogen ions accumulation which may affect lipid metabolism. HDL may be downregulated. The body must be in fasting but too long a fast and necessary catabolism , triglyceride-beta oxidation must not be induced if test results are to be valid.
For fasting blood glucose 8- 10 hrs are suitable. For Lipid Profile you need 12 - 14 hrs as recommended. Its important to clarify that Total cholesterol does not need fasting, Whereas, Fasting is mandatory for Triglycerides analysis. In addition, you can calculate LDL-C by Friedewald Equation ( [LDL-chol] = [Total chol] - [HDL-chol] - ([TG]/2.2) where all concentrations are given in mmol/L (note that if calculated using all concentrations in mg/dL then the equation is [LDL-chol] = [Total chol] - [HDL-chol] - ([TG]/5))
The Friedewald equation should not be used under the following circumstances: .
when chylomicrons are present
when plasma triglyceride concentration. exceeds 400 mg/dL (4.52 mmol/L)