I obtain a OD at 300 µg of my solvent extract equivalent to the OD at 800 µg of Gallic Acid (GA). After preparing the standard curve of GA, I was calculating the µg GAE/mg extract where the extract shows twice above the GA content in one unit. Here is the calculation...
That means, 800 µg GA is present in 300 µg extract,
so, 2666 µg GA is present in 1 mg of extract,
so, 2.6 mg GA is present in 1 mg of extract i.e. 2.6 mg GAE/mg extract.
Is the result justified? Then, what should be the possibilities?
Is there any reference publications with similar findings?
N.B. Near the same results have obtained in case of another assays.
Dear Tista
you can calculate the Gallic acid equivalent for your sample following equation
TPC = CV/m
Where C= concentration from calibration curve (mg/mL)
V= Volume of the extract used (mL)
m= Mass of the extract used (g)
Express your result as
TPC = mgGAE/g
Folin–Ciocalteu colorimetric method Singleton et al.
You need to prepare different concentrations of gallic acid solution in methanol. read the absorbance at 760 nm against blank. and plot the calibration curve. Then using the standard curve you can easily read equivalent amount in your extract
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