I am using 4 solvents in following order (n-hexane, DCM, Chloroform, Methanol, ) to get maximum phytochemicals for pharmacological activities.
Another question is in above which extraction is best soxhlet or cold extraction
Petroleum ether, chloroform, ethyl acetate and n-butanol
Use of different solvents during extraction process (polar and non-polar) will give you maximum phytochemicals.
Use n-hexane, ethyl acetate, methanol, and methanol/ water 70:30.
to prevent losing plant components ( low boiling point compounds) try to use cold extract ( maceration ).
for example: 200 g of dry plant ( separate parts) in 1000 ml n- hexane which immersed in an ultrasonic bath for 15 min. and then left at r.t. for 3 hr. under continuous stirring . decant the solvent .This process is repeated 3 times. the combined extracts is removed in a rotary evaporator...same procedure for other solvents.... don't forget TLC then separate using different Chromatographic methods ( flash chrom. is best).
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