I was processing the cells for SEM. I have done three times at different day's with same protocol and same concentration of fixative,2.5% glutaraldehyde. The first trail was done only for the control and the cells were very intact and smooth in SEM image. The 2nd time was done both for the control and treated cells, here the treated cells are more intact then the control ( not the expected result ). Third time was again done only with the control, here the cells are shrunken, and is different from the first control image.

What could be the possible reason for this problem ?

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