Hi everyone,
I am looking for any recommendation on concentrating very low molecular weight proteins (~1-3 kD). I would appreciate if anyone has experience in dealing with that low protein size. The downstream application would be for various immunological assays. Thank you.
Hi.
Maybe the best is to use a speed vac, a rotovac or lyophilizer...
Hope it works for you
Hi Mariam,
If you want to keep the protein as active as possible, I recommend using an ultrafiltration system with MWCO of 1KD or 3kD (Merck Millipore has related products) and using a lower centrifugal speed to ensure that the target protein does not pass through the membrane or aggregate. Of course, you can also use a dialysis bag with the appropriate MWCO range, but proteins may aggregate and precipitate during dialysis.
Hope it helps!
what is method to remove contamination or protect enviroment ???
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