My experiment is on cold tolerance of winter season grasses in field condition, and different NPK fertilizer levels are applied to optimize dose for coping winter stress.
Now i want to know Antioxidant enzymes activity will be higher in lower levels of NPK (where plants are very poor due to cold) or in higher levels of NPK (where plants are healthy)......
Dear Muhammad,
In most cases environmental stress such as cold, salt, Heavy metal etc,... induce ROS and AOS production in plant. One of the first mechanisms of the plant against ROS is to activate antioxidant enzymes like SOD, CAT, GR. According to your exp condition it is predicted that antioxidant enzyme activity in those plant that received stress must be higher than health plant due to higher ROS production.
In cool season grasses the complete fertilizer with emphasis on Phosphorus and Potassium is often recommended to improve over wintering. Phosphorus deficiency is most pronounced under cool winter conditions and is believed related to poor mobilization of P from the soil under that regime. The total antioxidant activity might relate to stress which the plants experience and to the fertility regime. Besides looking at the antioxidant levels it would be prudent to carefully develop an idea of the mineral absorption and look for the interaction of plant performance to these influences. Developing an experimental approach where the levels or nutrients are varied by treatments aimed at the concentration and or frequency of your fertilizer application and then doing both the plant performance, mineral composition and antioxidant assay might give better idea of the inter relationship. I agree with Ramin's comment that stresses can elicit and induce antioxidant elevation and wish you good luck in your endeavor. Being able to induce the increased antioxidant before stresses are applied can condition better ability to withstand the stress the proposed mechanism is stress is conditioned by tissue oxidation and the higher antioxidant ameliorates the negative consequences on the plant by allowing less damage.
Under cicumstance Nitrogen levels are repressing polyphenolics and the effect is depending on season, as in https://www.google.it/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwj83ozzltzOAhVIiRoKHQKzBCEQFgghMAA&url=http%3A%2F%2Fwww.southwestern.edu%2Facademics%2Fbwp%2Fpdf%2F2008bwp_niemeyer-vol8.pdf&usg=AFQjCNG7DaReRJyEqzB8MVJ0sfTSk_35cw&sig2=X8s1-fcFGmgyCqpdRbkR-w
Then one should apportion any response to growth rates under specific and selective nutrint stress. This stress often requires a suitable artificial media in containers, because P and K levels can be affected by mineral slow release and mobilization. With best whishes,
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