What type of filter should i use to obtain folate-free plasma?

Matteo Della Porta @Matteo-Della-Porta

14 February 2020 1 1K Report

I have rat plasma EDTA and I need plasma free folate.

I read from Chandrika J Piyathilake et al. Analytical Chemistry Insights 2007: 2 that I need a 0.22-micron filter.

Which of the following filter material I should use?

1. cellulose acetate membrane polypropylene housing

2. polyethersulfone membrane polypropylene housing

3. PVDF membrane polypropylene housing

4. polystyrene housing regenerated cellulose membrane

5. polypropylene housing regenerated cellulose membrane

6. Nylon membrane polystyrene housing

7. PTFE membrane polypropylene housing

8. alumina-based membrane polypropylene housing.

Mirko Marino

Nylon: General laboratory filtration; Filtration for most HPLC samples

PTFE: Filtration of aggressive organic, highly basic, or hot solutions, transducer protectors; Filtering aqueous solutions after prewetting with an alcohol

PVDF: General biological filtration; Filtration of samples where high protein recovery is desired

PES: Ion chromatography; Tissue culture, proteins, and nucleic acids filtration; High-temperature liquids

GMF: Dissolution testing; General filtration

Polypropylene: Filtration of biological samples; Filtration of aggressive organic solutions

Cellulose Acetate: Tissue culture media filtration, sensitive biological samples

Source: https://www.thermofisher.com/it/en/home/industrial/chromatography/chromatography-sample-preparation/sample-preparation-consumables/syringe-filters.html?gclid=CjwKCAjw7-P1BRA2EiwAXoPWAwiNb04KeNGMYF1j0Fb1_VzXVID4Y-LTNpEA_cGQnvRw0-_5EPbm-BoCyHwQAvD_BwE&ce=E.20CMD.DL101.29346.01&cid=E.20CMD.DL101.29346.01&ef_id=CjwKCAjw7-P1BRA2EiwAXoPWAwiNb04KeNGMYF1j0Fb1_VzXVID4Y-LTNpEA_cGQnvRw0-_5EPbm-BoCyHwQAvD_BwE:G:s&s_kwcid=AL!3652!3!383099655783!p!!g!!syringe%20filters

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