Hi,
I'm developing a targeted nanoparticle for drug delivery purposes where I will use anitbodies/antibody fragments as targeting devices.
As a step to quantitate the amount of antibody bound to my nanoparticles I was thinking of using an ELISA for this purpose I had two questions:
1) What kind of ELISA should I choose? A direct or sandwich ELISA?
2) I'm using a rabbit anti-human antibody (or fragments) of IgG isotype for targeting, what antibodies should I choose for detection and for capture (if sandwich)?
Thank you in advance!
Hi,
I think you have to use sandwich ELISA, and you can use anti-guinea pig immunoglobulin conjugated with horseradish peroxidase.
Hello! I Think that is easier the direct ELISA for the detection of antibodies, you only have to buy an antibody that recognise the specie in which you´re working. Like Murat said use an inmunoglobulin conjugated with horseradish peroxidase. I use the ELISA sandwich for antigen detection, not to antibody. Have a nice weekend!
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