Could anyone recommend the appropriate anesthetic procedure for BMT in mice?
I do bone marrow transplantation in mice as a part of my study.
The problem is, the engraftment rate got pretty low recently.
I suspect that it's due to the change of the drugs.
I'm currently using the mixture of three drugs, which are midazolam, medetomidine and butorphanol.
I used to use the combination of pentobarbital(i.p.) and sevoflurane(inhalation).
Would it be possible that it's because of this change ?
I do transplantation by retro orbital injection.
I know it's possible by tail vein injection with no anesthetic drug usage. I tried, but it was technically difficult for me.
I appreciate any suggestions.
Thank you.
I would suggest that you read the attached papers. I would expect that your bone marrow transplant is likely to be so stressful for mice that a lot of them would simply die on account of the stress reaction induced by the procedure. Perhaps the best approach would be to perform spinal analgesia on the mice before performing your injection. That would be less than ideal, but far more effective than anesthesia. Good luck and let me know if you have questions.
Lewis S Coleman
Thank you very much for your suggestion.
I'll check out the papers which you showed.
I'm just wondering, if it's easy enough for me to perform spinal analgesia for retro-orbital injection, which I think means that I need to block the facial nerves.
Thank you again for your opinions.
Morin to do the pain control , propofol to induction and Isoflurano to keep The anesthetic plan
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