thank you very much Masoumeh Foroutan Koudehi

Hello Naser,

It is always better to use Pet-ether or n-Hexane and even di-ethyl ether also are useful remove oil from the extract.

It is always better to de-fat to remove oil from the herbs and/or of raw plant materials which are proceeding for extraction.

You can use Hexane or Pet. Ether or Diethyl Ether in a liquid-liquid or liquid-solid extraction.

n-hexane is better option!!!

thank you Bruce Neagle Paroma Arefin Venkata Narasimhaji

My pleasure

For oil (essential oil) I will utilize a solvent with a boiling point between 60-70C, like n-hexane or pet ether. A mix of chloroform-methanol can be effective as well depending on your extract.

Hexane

Pet-ether or n-Hexane

good answer Naser Jawad Kadhum Harith Rajab Almosawy Dhifaf Zeki

thank you Ali Hassan Abood

thank you very much Masoumeh Foroutan Koudehi

Chloroform, Hexan

* This process utilizes petroleum solvents such as petroleum ether, hexane or toluene; alcohol solvents such as methanol or ethanol; or carbon dioxide.

* By introducing the suspension to a low heat (below the boiling point of the solvent). This allow more oils to dissolve into the solvent.

thank you very much Rana Alqarraawi Dhifaf Zeki Masoumeh Foroutan Koudehi

good idea

Dear Naser, antioxidant and antibacterial assay. P. minus was extracted using maceration method (solvent extraction) to study the effect of solvent type and concentration in biological activity of P. minus. Seven solvents (water, 50% (v/v) methanol, 50% (v/v) ethanol, 70% (v/v) methanol, 70% (v/v) ethanol, methanol and ethanol) were used in order to select the best solvent to be used in supercritical fluid extraction (SFE). The antioxidant capacity was evaluated using four different assays: Total Phenolic content assay (TP), Total Flavonoid content assay (TF), Ferric Reducing/antioxidant Power (FRAP) assay and free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Seventy percent methanol extract showed the highest polyphenol and flavonoid content. The highest TP and TF were from 70% methanol extract (11.3±0.06 mg gallic acid equivalents/g extract (mg GAE g-1) and 22.5±0.07 mg catechin equivalents/g extract (mg CAE g-1 extract, respectively). Seventy percent methanol extract also showed the highest FRAP value and percentage of DPPH radical inhibition which were 377.6±5.40 μMol Fe (II)/g sample and 80.3±0.10%. The high antioxidant capacity was significantly correlated with high TP/TF content. Agar-well diffusion method was used to investigate the antibacterial activity where result showed that 70% methanol extract exhibited the largest inhibition zone against Staphylococus aureus (Gram+), 16.2±0.30 mm in diameter.

Regards

I think that any chemical material that is used will harm the grass and for this must be used biological solutions or a substance that does not harm the grass and does not remain in the soil

I think we should repeat the extraction procedure with petroleum ether to remove oil.

best work

By introducing the suspension to a low heat (below the boiling point of the solvent), This allow more oils to dissolve into the solvent.

antioxidant and antibacterial assay. P. minus was extracted using maceration method (solvent extraction) to study the effect of solvent type and concentration in biological activity of P. minus. Seven solvents (water, 50% (v/v) methanol, 50% (v/v) ethanol, 70% (v/v) methanol, 70% (v/v) ethanol, methanol and ethanol) were used in order to select the best solvent to be used in supercritical fluid extraction (SFE). The antioxidant capacity was evaluated using four different assays: Total Phenolic content assay (TP), Total Flavonoid content assay (TF), Ferric Reducing/antioxidant Power (FRAP) assay and free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Seventy percent methanol extract showed the highest polyphenol and flavonoid content. The highest TP and TF were from 70% methanol extract (11.3±0.06 mg gallic acid equivalents/g extract (mg GAE g-1) and 22.5±0.07 mg catechin equivalents/g extract (mg CAE g-1 extract, respectively). Seventy percent methanol extract also showed the highest FRAP value and percentage of DPPH radical inhibition which were 377.6±5.40 μMol Fe (II)/g sample and 80.3±0.10%. The high antioxidant capacity was significantly correlated with high TP/TF content. Agar-well diffusion method was used to investigate the antibacterial activity where result showed that 70% methanol extract exhibited the largest inhibition zone against Staphylococus aureus (Gram+), 16.2±0.30 mm in diameter.

I follow

follow

I suppose the two fase separation using polar/nonpolar solvents is efficient

Petroleum ether

Hexan

good answer Farah Al-Mamoori Raghad Sahib Hussein Hassan Ruaa SH Redeiny Intisar Razzaq Sharba

good answer

Agree with Hussein Hassan

nice question

Agree with Hussein Hassan

Dear colleague

Saponification of oils can be used efficiently.

regards

je pense qu'il faut bien délipider avec ether de pétrole ou hexane avant d'entamer l'extraction avec votre solvant. apres extraction je suppose qu'il faut ajouter a votre extrait un des solvants précedents et laisser décanter en deux phases.

good evening

dear dr. according to my past research

petroleum ether is the best

best regrds

I think Hexan is good

nice question

Dear friend,

You use pertroleum ether

Petrolum ether

Yes... petroleum ether is the best

good question

Methanol is the most commonly used extraction solvent due to its high polarity which could produce high extraction yields.

Ethyl Alcohol is the best solvent

Dear Dr. according to my past research!

Firstly, you may de-fatting the total lipids from sample by extraction it (Methanol: Chloroform 1:2 V/V) according to cold method for oil extraction (Folch, 1957 assay), or Petroleum ether separately. Then you extract the bio-active substances in herbs by many solvents e.g. Ethanol or Methanol or 70% of these alcohol (70:30 Alcohol: distilled. H2O).

You have to use a mix of chloroform-methanol .

Regards!