I have matrial FeO containing graphitic carbon from MOF Fe and when I did TEM measurement:
1. I using big consentration between my FeO and ethanol and then sonication for 5 min, and the result of TEM the particles so agrigated, until I can’t see my FeO lattice because covered or obstructed with my graphitic carbon layer and my feo is agrigated from one with each other until i cant sure the particle size of my FeO
2. I add surfactant and sonication 15 Min but with smaler consentration and the result i just see a little amount of particle and still agrigated and the carbon and my FeO in different position so cant showed the carbon lattice and FeO lattice together
what the best way for preparing my TEM sample if i wanting my compound not soo agrigated but still showed many material on there...? Thanks you all
That's a good question. The samples are a powder. The powder is applied to a metal lattice.The surplus powder is blown off.
Dear Mr. Maulana Achmad ,
I agree with you in using very small amount of sample and added it to ethanol or methanol or water, and then applied a ultrasonic for about 3 min, now, you transfer one drop from solution on clan glass slide, and wait to dry it and store in plastic dish after that send to TEM analysis. ( notice: must prevent the pollution of sample in dust or CO2). from other hand, th using different solvent to prepare a colloid from sample is vip to study the particle size that can be compared with the result which produce during physical adsorption (BET) to calculate surface area and particle size by depended on some calculations.
Best regard,
Thanks for your respond..
and for Luma M. Ahmed, I want to ask you what did you do for sampling to your grid? is that droped your sample directly to your grid and wait until dry or you droped your sample first and placed your grid above that drop and wait until dry?
The best method is droped your sample on glass slide and wait dry it in his place ( vaporization of solvent) then measure in TEM.
Best regards,
Dear colleague Maulana Achmad
Have a nice time and good day.
You can make your material as thin film for TEM as follows:
1- Deposit a salt layer on the substrate
2- Deposit your material on salted substrate (up to max. 70 nm)
3- Use a suitable angle to merge slowly your thin film sample in distilled water; salt layer will be solved and the film floats the you can pic the film on your TEM grid.
I wish this is useful and help you.
Yours
A. EL-Denglawey
Ultra sound processing changes the surface properties of the original sample. On the translucent detector of the microscope, you see already another morphology. It's enough just ethanol and time to remove it.
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