I'm having trouble to analyze the data I collected from experiments.
In my experimental design I'm testing how sound intensities and interval between sound stimuli influence the reflex response in rats.
In each experimental session I expose the rats to different intensities of sounds (three levels of intensities) with a fixed interval between the presentation. In the first session, I used a fixed interval of 10 second, and one week later I tested the same animals but with an interval of 20 seconds between the stimuli. I can't use mixed intervals, that is, I need to use either 10 seconds interval or 20 seconds interval.
I'm not sure what statistical test I should use to compare the response from the rats between the condition with the 10 seconds interval to the condition with 20 seconds interval. I tried to use the repeated mesure ANOVA in JASP (0.10.0.0), but it seems as if it was comparing 12 rats, not the same 6 animals I used in the entire experiment.
I did also a second experiment in which I varied the intensities of the sounds. In the first week I used intensities L1, L2 and L3 (three levels of the same factors) and a week later repeated the session but with intensities L4, L5 and L6 (again three different levels). But I'm not sure if i need to treat those intensities as 6 levels of the same factor (sound intensities) or if i need to treat them as just three levels of the same factor and compare the results got from each experiment. But if I did so, I have the same problem that the JASP seems to be analysing as if I had 12 rats and not just 6. Again, I can't use mixed sound presentation, I need to use either the first configuration (L1, L2 and L3) or the second configuration (L4, L5 and L6) in the experimental session.
Is that correct? Or should I use another test to compare those results?
I am not familiar with your analysis package, but I think you should be using a one-way repeated measures ANOVA, assuming you do not violate the defined assumptions. If you do you should then consider using non-parametric statistics.
- Badges
- Science topic