I need to induce LTP in hippocampal acute brain slices (rodent) and measure extracellular fPSPs.
Different bipolar stimulation electrodes available on the market have different impedances (0.1 MOhm, 0.5 MOhm, 1Mohm, 1.5 MOhm, and 2 MOhm) and I am not sure about the most suitable (no info from the customer service…).
Could anyone recommend a particular impedance based on experience?
Hi Bruno. We use platinum/iridium bipolar (matrix) electrodes from FHC for hippocampal recordings and LTP. They have impedances in the range of 200 - 500 kOhm, so your options of 0.1 or 0.5 MOhm should work fine I imagine. The optimal resistance might also depend on the design of your stimulus isolator, so it might be worth checking the manual for that or contacting the manufacturer.
Hi there. I use 0.1 MOhm tungsten electrodes. Using 0.5 MOhm or higher impedances didn't give good ltp results with my recordings. Good luck.
I use concentric bipolar electrodes from FHC CBAPC75! don't know the impedance but they work just fine!
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