I used MeOH and water for extracting natural products from bacteria but active fraction did not misible in water methanol solvents for HPLC analysis. How can I prepare HPLC sample for for analysis? Is there any alterative solvents?
please indetfy the type of natural product ( alkalois, phenolics,glycosides/ macrolides or others) based on that you chose the solvent for extractions and HPLC