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Questions related from Mohammad Nazrul Islam Bhuiyan
When we cultured in bacteria by adding growth promoting factors for rapid growth, what happened in this time. At that time bacteria growth/size are increase or both. Please any one can explain in...
02 March 2015 1,509 8 View
My experiment requires totally oxygen free environment, how can this get? I used a reacted mixture solution in physilogical conditions.
20 September 2014 9,752 17 View
I am trying to isolate active compounds from bacteria. I have used an ODS HPLC column but the active compound did not elute within this column. Can anybody give me any suggestions? My compound is...
19 March 2014 639 4 View
I'm having a problem separating a single compound using 100% water as a mobile phase in HPLC (ODS). My compound is more polar and it is eluted within 3 minutes when I used 100% water as a mobile...
07 March 2014 9,976 17 View
I need a protocol of cosmosil 75 C18-OPN open column (Nacalai USA, Inc,) or published articles.
07 March 2014 2,999 0 View
I am trying to isolate an active compound from an HP20 water flow fraction by HPLC. This compound is not bound in HP20 resin or an SPE (solid phase extraction cartridge). When I inject this sample...
05 March 2014 3,988 3 View
Sometimes we use a huge amount of solvents for extracting our targeted molecules. Some solvents are so expensive and for this we recycle this solvent for further use to save our money. But one...
24 July 2013 3,751 9 View
My targeted small molecule is difficult to separate through HPLC. I collected active fractions through pre-parative and analytical HPLC, but this is a mixture of more than 5 compounds. Now I want...
22 July 2013 1,706 16 View
I am trying to separate my active compound through HPLC. When I injected my samples, I get more than 20 peaks and I was collected all peaks into 10 different fractions. After doing bioassay, I...
20 July 2013 7,215 1 View
19 July 2013 8,599 6 View
There are a number of factors that control the number of injections any modern HPLC column can withstand. Some of the factors are based upon the mode: reversed phase, ion exchange, size exclusion,...
17 July 2013 3,846 6 View
My target compound is a mixture of three compounds but it is about 80% pure (goal compound). The other two compounds in this mixture are minor. Is it possible to identify my target compound...
10 July 2013 6,954 2 View
I am trying to isolate a growth factor from bacteria but when I used HPLC to isolate this compound, it gave three peak in different retention time. Why? Please give me some suggestions. Thank
09 July 2013 5,721 9 View
I only have a 0.5 mg pure sample. This amount I got from more than 66 litres of culture broth. I am worried, is it sufficient for an NMR analysis? My sample is isolated from bacteria and it is a...
09 July 2013 9,222 22 View
I need the total procedure to identified aromatic compounds with the help of Retention Index.
04 July 2013 1,845 21 View
I used MeOH and water for extracting natural products from bacteria but active fraction did not misible in water methanol solvents for HPLC analysis. How can I prepare HPLC sample for for...
03 July 2013 3,908 7 View
03 July 2013 1,407 1 View
