Im looking for a software to plot more than 20 circular genomes aligned at the same time. I had experienced with Blast Atlas, but it seems not be avilable anymore.
I need something very user friendly :)
Suggestions?
thanks
Hello Dr. Thiago,
Try BLAST Ring Image Generator (BRIG), where you can map large no. of genomes (draft or complete) as queries on a reference genome.
http://brig.sourceforge.net/
http://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-12-402
Good luck!
BRIG, works fine and is easy, the problem is that you have to ensamble your contigs in a single supercontig (chromosome), but the good thing is that you can use medusa web server (http://combo.dbe.unifi.it/medusa/) or CONTIGuator to do it, both use a genome as reference. After you have your superconttigs you can use BRIG !!
Thank you guys! I will try to work on this next wee, then I can give you a feedback.
Hi guys! Im facing some problems to run BRIG.
I downloaded Blast+ and the BRIG, choose the final destination of the files but I wasnt able to run it. A message: couldnt run blast, please check it it is installed and BRIG's BLAST location is correct" and I couldnt fix it yet....
Do you guys have an idea what is going on?
You have to give the path of your blast file.
The correct way is, go to Preferences --> BRIG options and then add the path of blast in BLAST binary folder; I think that is your problem, maybe you are adding the path in the main window (blast option).
I hope it helps !!!
I already tried it before and still doesn´t work... My main BRIG folder in in the desktop area. Is it a problem?
maybe it if the permission of blast, I don't know how is it in Windows, but in linux and mac, you have to open the terminal and change the directorio to blast:
cd path/to/blasfolder/ (the path)
and then use chmod
chmod 755 blast (binary file)
after that add the path to BRIG and try again !!!
it do´not matters where the BRIG folder is, if woks fine in any folder !!!
Hi Thiago,
Apart from path settings, this also happens when you have more than one BLAST/BLAST+ installed in your system or if your blast is not properly installed. Remove all and install new. Then try.
Problem solved! I ran the examples and from this point I will star to work with own dataset.
Thanks a lot for the informations
Hi! Just here to share that I had the same error while running BRIG - "couldnt run blast, please check if it is installed and BRIG's BLAST location is correct," and I found out that BLAST was not running because a .dll file was missing. So I installed the file, and it ran perfectly after that. The file is VCruntime140_1.
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