Looking for an easy/efficient way to internally calibrate mass spectra.
There is a software called ReCalOffline that is installed on a lot of computers running Orbitraps - not sure who makes it but it works well!
I have been using mmass (http://www.mmass.org/). Mass spectra can be opened after transformation of .RAW files, for example into mzML files, and then be recalibrated with own reference lists. Unfortunately the software is not updated anymore.
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
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24 June 2024 9,319 0 View
Hi, In a previous question I asked how to effectively model springs in Abaqus. However, I figured out the feature under Assembly -> springs/dashspots -> node to node Springs. I have a cylinder...
20 June 2024 892 3 View
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17 June 2024 8,165 7 View
I'm interested in the earliest days of placental terrestrial locomotion in large herbivorous taxa. Phenacodus seems to be transitional in this regard. Any others?
06 June 2024 5,182 2 View
Hey everyone! I'm doing peptide synthesis on Rink amide resin and sometimes I need to add amino acids with secondary amines or beta amino acids. Normally I synthesize 35 to 40 mer peptides. As a...
21 May 2024 9,035 0 View
Sometimes TCSPC curves are analyzed using the actual photon counts, and sometimes they are normalized with the signal running from 0 to 1, or even from 0 to some value less than 1. What quantity...
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I am currently using CASTEP version 22.11, and I encounter the following error when running calculations with spin-orbit coupling: Error with dsytrf in nlpot_prepare_precon_ks Current trace...
19 May 2024 4,236 5 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
EDX analisys
31 July 2024 5,947 2 View
I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2...
30 July 2024 8,771 3 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
Dear all, I have noticed, as the ZnO content increases, the Raman intensity at 100cm-1 also increases. However, there is a peak nearby at 130 cm-1, which I could not find in any paper related to...
28 July 2024 5,410 1 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View