What sizes are the fragments of reduced albumin?

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Omar Gonzalez-Ortega

Albumin does not have disulfide bonds, commercial albumin is contaminated with antibodies and transferrin.

Rita Hargitai

Thank you for your answer! So the many bands I got are from contamination? Actually, I can see bands at 100, 80, 60, 50, 42, and 17 kDa.

You should see an intense band around 67 kDa, which is albumin, along with fainter bands of contaminants.