Rita Hargitai

11 Questions 7 Answers 0 Followers

Questions related from Rita Hargitai

Which mouse can be used better for the study of respiratory sensitization/asthma: BALB/c or BALB/cByJ?

We would like to order a BALB/c strain from Charles Rivers or Jackson laboratories, but apparently there are two such strains, and we are not sure which one is better or if there is any difference...

10 November 2023 5,933 0 View

What sizes are the fragments of reduced albumin?

We used a reducing agent (BME) in Western blot, and there are several bands in the gel. I wonder what sizes could we expect for albumin. Thank you for your answers!

21 October 2022 6,509 3 View

Can you isolate RNA from mouse blood with QIAamp-RNA Blood Mini kit ?

According to the protocol, this kit is designed to human blood, but I wonder if I could use it with mouse blood. Could there be a difference in the amount of blood required? Has somebody used it...

16 March 2022 1,953 2 View

How can I get access to NormFinder, GeNorm or BestKeeper softwares?

I would like to compare reference genes, but I could not find the webpage from where I could download these applications. Thank you.

15 February 2022 2,063 4 View

Why are all Ct values lower in the samples of one group than in the other?

I would like to compare gene expression in samples of leukemic mice. I found that Ct values are consistently lower in one type of leukemia than in the other. I have tried several housekeeping...

02 June 2021 2,509 3 View

Why do we get signals in both colours in FISH?

We use one probe with orange (Alexa555) dye to bind to the centromere of one chromosome and another probe with green (Alexa488) dye to bind to a gene. But in some nuclei we can see 5-10 spots that...

17 July 2019 3,019 2 View

What could be the reason that we don't get specific signals in FISH, only non-specific signals in the background?

We use FISH with commercial probe and hybridisation buffer and with mouse bone marrow cells. We have interphase and metaphase nuclei as well. We use 72 C for 2 min for co-denaturation, then 24 h...

04 July 2018 7,937 5 View

Can denaturation of slides in FISH (in situ hybridization) be performed with normal formamide (not deionised)?

We have performed the denaturation in FISH with normal formamide (73 C), but it seems that the probes did not attach to the chromosomes, there are only non-specific signals. I wonder whether it...

22 June 2018 1,021 3 View

How to normalise to mtDNA copy number when examining mtDNA deletions?

MtDNA copy number is increasing with treatment, but deletion number seems to decline. Is it correct to normalise only to mtDNA copy number or should I somehow take into account that it increases...

12 August 2017 6,268 4 View

What could be the reason for highly variable Ct value differences in a diluteion series of qPCR?

I performed a qPCR with a dilution series (1:2; 1:4; 1:8; 1:16) to calculate amplification efficiency of the target. My results showed that between two points the difference in Ct values were:...

25 May 2017 6,637 0 View

Does the linear mixed effects model tolerate a dependent variable that has multinomial distribution instead of normal?

I have an ordinal multinomial variable that shows a normal distribution by looking at it, but the K-S test is significant and it has categories. My statistical software (SPSS) could not calculate...

14 October 2015 2,330 9 View