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Questions related from Rita Hargitai
We would like to order a BALB/c strain from Charles Rivers or Jackson laboratories, but apparently there are two such strains, and we are not sure which one is better or if there is any difference...
10 November 2023 5,829 0 View
According to the protocol, this kit is designed to human blood, but I wonder if I could use it with mouse blood. Could there be a difference in the amount of blood required? Has somebody used it...
16 March 2022 1,916 2 View
I would like to compare reference genes, but I could not find the webpage from where I could download these applications. Thank you.
15 February 2022 2,027 4 View
I would like to compare gene expression in samples of leukemic mice. I found that Ct values are consistently lower in one type of leukemia than in the other. I have tried several housekeeping...
02 June 2021 2,447 3 View
We use one probe with orange (Alexa555) dye to bind to the centromere of one chromosome and another probe with green (Alexa488) dye to bind to a gene. But in some nuclei we can see 5-10 spots that...
17 July 2019 2,986 2 View
We use FISH with commercial probe and hybridisation buffer and with mouse bone marrow cells. We have interphase and metaphase nuclei as well. We use 72 C for 2 min for co-denaturation, then 24 h...
04 July 2018 7,897 5 View
We have performed the denaturation in FISH with normal formamide (73 C), but it seems that the probes did not attach to the chromosomes, there are only non-specific signals. I wonder whether it...
22 June 2018 993 3 View
MtDNA copy number is increasing with treatment, but deletion number seems to decline. Is it correct to normalise only to mtDNA copy number or should I somehow take into account that it increases...
12 August 2017 6,230 4 View
I have an ordinal multinomial variable that shows a normal distribution by looking at it, but the K-S test is significant and it has categories. My statistical software (SPSS) could not calculate...
14 October 2015 2,291 9 View
