When available, are you planning to receive SARS-CoV-2/COVID-19 immunization? What do you most fear about it?

If possible, let us know about the which vaccine would be available in your geographic location.

06 December 2020 3,527 23 View

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

05 November 2020 8,841 3 View

Checking for overfitting in niche models?

In niche models, overfitting has been traditionally assessed by large difference between training and test AUC or other model performance metrics. Are there other methods to assess model...

07 October 2020 9,060 3 View

How do we balance the argument on the effect of hydroxychloroquine in Covid-19 prophylaxis and treatment?

Hydroxyl-chloroquine has been listed as a potential drug in the management of Covid-19. The effect of this drug in Covid-19 prophylaxis and management is trending on social media. The Doctors...

29 July 2020 2,552 4 View

Positive value in NCT real-time PCR?

Hi I will be happy for your help. I'm doing real-time PCR with two primers called MT-TL1 and 18S, for mtDNA and houskeeping gene, and I got positive control on both NCT. I tried also different...

07 March 2020 3,953 4 View

Can anyone help with a detailed assay procedure for the spectrophotometric measurement of protein carbonyls in tissues?

Protein carbonyls are important compounds to measure progression of oxidative stress in tissues. Available articles online do not have details about how to do final estimation/calculation of the...

06 February 2020 2,897 8 View

Suction pump of the Rotary evaporator?

Can someone explain why the suction pump of the rotary evaporator sucks solvent from the condensation chamber, also pumping out engine oil when it's being switched off and perhaps the solution to...

19 June 2019 3,530 13 View

Where can i purchase tail cuff device?

Anyone with the idea of where to buy tail cuff device for measuring the blood pressure of rats. Appreciation in anticipation. Fasan

10 April 2019 2,700 2 View

Defat before phenolic rich fraction extraction?

06 April 2019 2,866 8 View

Hello All, does the sum of five linkert variables become a continous variable or does it still remain categorical?

I summed up five linkert variables measuring satisfaction with life scifea+,scifeb, scifec+, scifed+, scifee...I want to run a bivariate regression with the sum of these fiver linkert variables as...

19 January 2018 8,695 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View