I was lucky to isolate a strange bacterium from salt marsh detritus. Its 16S rRNA gene sequence (~1350 bp I got) only shares ~ 90% similarity to those of Tunicatimonas and Porifericola which are belong to Bacteroidetes phylum. This bacterium grows slowly and forms rough colonies with pink color under aerobic condition. It can degrade xylan and cellulose.
Congratulation to you! First replicate the 16SrRNA analysis to sure the result (more than 1500 bp). If your result is replicate, you do not need to do DNA/DNA hybridization test to complete identification the bacterium. You can simply do biochemical and morphological tests. Please consider, you may isolate new Genus! To obtain best result, study Bergey's Manual of Systematic Bacteriology. Good Lock!
Bacteroidetes are an important group of bacteria in animal guts involving the degradation of complex carbohydrates. Most of them are anaerobic.
If you plan on publishing a paper in IJSEM you will need to run a suite of analyses
- lipid analysis
- peptidoglycan
- substrate range
- enzyme test strips (... forgot what those are called)
- growth temperature optimum
- pH range is useful
DNA:DNA hyb is still used, but pretty useless in my mind. For the same price you can sequence it's genome.
My suggestion is to work with someone who has described strains for IJSEM before, if you have never done it. It's more technical than it might seem.
you can do G+C content analysis, cellular fatty acid profiling, some group specific physiochemical characterization, and for more confirmation may be DNA-DNA hybridization. then you can say belongs to which genus/species.
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