Hello everyone. I am working with SH-SY5Y neuroblastoma cell line recently. 48 hours later, the incubator stopped working during the night. This conditioned resulted in reduction of confluncy from 70% to 30%. Again, this happedned the day after unfortunately and now the cells are in a terrible condition. I attach a picture of them here. How I can recover them?
This the medium I am using:
Non-essentional amino acid 1x
Gln 2 mM
FBS 10%
Gentamicin sulfate 50 ug/ml
Ham's F12:EMEM (1:1)
Thank you in advance.