Mona Abdollahi

11 Questions 18 Answers 0 Followers

Questions related from Mona Abdollahi

Can anyone provide a detailed Congo red staining protocol for detecting amyloid aggregates in neuroblastoma cell culture please?

I plan to seed neuroblastoma cells. Differentiate them with retinoic acid, treat them with AB1-42 and then stain them with Congo red to observe amyloid aggregates in those cells. Thank you in...

21 January 2019 340 3 View

Why we can't use absolute quantification method to compare the changes of gene expression using qRT-PCR?

Hi. An example is when cells are treated with different concentrations of a drug. To compare the treated samples and calibrator (non-treated cells) can we do as followed? 1. generating a standard...

04 November 2018 724 3 View

Do you have a detailed protocol for dissolving Abeta25-35 in DMSO?

Hello, I have no access to HFIP, and I already tried NH4OH method with little success. The ThT assay resaults did not make sense by this method. And AFM showed the 500 nm spheres were formed. Any...

04 September 2017 4,389 2 View

For peptide concentration determination, measured molar extinction coefficient or calculated one?

Hey, There are some calculation you can predict the amount of epsilon (molar extinction coefficient) by amino acid content. You may also prepare a solution of the protein of interest with known...

04 August 2017 5,573 2 View

Does the choice of solvent affect the molar extinction coefficient of a particular peptide?

Hi everybody, I am trying to determine peptide concentration by far-UV spectroscopy and I was wondering if different solvents (water, ammonium chloride,Ham's F-12 medium and PBS) that I use as...

30 July 2017 1,329 2 View

What is the stability and half-life of amyloid beta 25-35 fragment?

Hi everyone.I considering different solvents for preparing this fragment. NaOH and ammonium hydroxide are my choices instead of HFIP. I like to know how much time I can store them in -20 or -80...

27 June 2017 2,845 4 View

What should I do to recover unhealthy cell culture?

Hello everyone. I am working with SH-SY5Y neuroblastoma cell line recently. 48 hours later, the incubator stopped working during the night. This conditioned resulted in reduction of confluncy from...

04 June 2017 9,213 4 View

For transferring cell lines between laboratories in 2 hours distance should I keep the T-flask vertical or horizontal during communication?

The cell line is SH-SY5Y neuroblastoma.

14 February 2017 5,190 6 View

Why in Stewart phosphate assay a reverse trend is observed? I mean why by increasing amount of phospholipid liposomes, OD decreases?

I have liposome of egg yolk PC in PBS, 0.01-0.2 mg/ml I obtained a precise standard curve by PC solvent.

28 August 2016 1,216 0 View

What is the best protein assay selection for a peptide as small as 1000 Da?

Which method is the best choice to determine protein concentration of amyloid beta peptide (25-35) with a molecular weight of 1060.28 g/mol? Is Bradford assay incompatible because it responds to...

01 January 1970 560 8 View

Can the venue for IELTS speaking test be selected by test takers?

Can my IELTS speaking section be scheduled on a separate location or does it have to happen on the venue along with the other modules?

01 January 1970 267 5 View