1. Check existing literature for genes related to salinity, preferentially those related to salinity in wheat.
2. Identify which genes have already been used to generate transgenics and determine the success (or not) of each.
3. Identify target gene(s) of interest you will use.
4. Decide on vector construction methods and delivery. That is, determine which promoter and terminators to use. Do you want a constitutive promoter or inducible one? Which promoters are available to you? Where do you want the gene to express? Will the gene be expressed in the nucleus or chloroplast? How will you deliver the gene into the plant (Agrobacterium or Gene Gun)?
5. Identify and test transformation methods to generate transgenic plants? Use an existing or new protocol?
6. Generate transgenic plants. This will take a while, consider the length of time required.
7. Use literature to identify ways to screen salinity tolerance.
8. Develop and practice method for screening for salinity tolerance
9. Screen transgenic progeny
10. Evaluate project. Is the transgenic successful? What can be improved?
Craig Schluttenhofer Thanks sir, for a timely and comprehensive response. Regards from India.
Article Improving salinity tolerance in crop plants: A biotechnological view
https://www.tandfonline.com/doi/abs/10.1080/07352689.2013.758544?src=recsys&journalCode=bpts20
http://www.indiaenvironmentportal.org.in/files/file/Salinity%20tolerance%20in%20plants.pdf
Article An efficient and reproducible protocol for the production of...
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