preparation of column chromatography for isolation of plant compounds. how much silica should be packed in glass column for 1 gram of plant extract
I tend to use 2-5% crude extract by weight. For 1 gram crude extract, I would use about 20 g silica, and I would also dry load it.
Thanks for your valuable suggestion,
Sir, if you could explain " 2-5% crude extract by weight."
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