I want to fix a dose for antioxidant activity on zebra fish. In the above mentioned dose, which one will be more reliable? Or should I follow some other dose pattern?
For expressing antioxidant activities, we usually use the concept IC50. In addition, a positive control should be used as a comparison. The optimal dose may be the IC50. For the specific definition of IC50, you can see my paper published in Food Sci & Biotechnol.