What should be the exact ratio of alcohol and water for hydroalcoholic extraction by soxlet ? Among methanol and ethanol which is recommended ? Ref ?

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Which hurdle/ factors mainly restrict the free access to research papers ?

10 November 2018 3,329 8 View

Which factor/factors makes the flower colour differentiation in the same species ?

As we see the Clitoria ternetea like species which are found with two different colored flowers white and blue.Which factor/factors makes the flower color differentiation in the same species ?

10 November 2018 9,444 3 View

Is mentha oil fumes safe for lungs ?

Is mentha oil fumes safe for lungs ? Whether it exhibits toxicity not at all ?

10 November 2018 353 4 View

How long the Soxhlet extraction Should be be run in a specified solvant ?

I want to use Soxhlet method for the successive extraction of a plant material. but I don't know how long the Soxhlet extraction Should be be run in a specified solvant. i.e If I started with...

08 September 2018 9,011 5 View

What is the success rate of "Request full text" in terms of getting paper ?

What is the success rate of "Request full text" in terms of getting the desired research paper in RG ?

01 January 1970 1,809 5 View

Had Pharmacovigilance proved to be relevant for Ayurvedic drugs yet ?

Had Pharmacovigilance proved to be relevant for Ayurvedic drugs yet ? Has these cases been reported and resolved upto a significant level ?? Specifically in India ??

01 January 1970 8,833 9 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View