I am injecting rRNA transcribed by mMESSAGE mMACHINE T3 Transcription Kit into Xenopus oocyte for 2 electrode recording. I am injecting 1ul per egg. What concentration is can be a start for this type of injection?
It depends on what you are injecting & where you anticipate its expression. Check out Xenopus methods here...
http://wiki.xenbase.org/xenwiki/index.php/Protocols
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I am using gelatin to model mechanical properties of brain tissues in-vitro. Do you have any suggested concentration ? Thank you so much.
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Methods are based on Chel-Guerrero and colleagues'Phytochemical Profile, Toxicity, and Pharmacological Potenti... . We use a 1M Tris buffer with pH of 6.8, with Bovine Serum Albumin as the...
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