IHC perfused brain tissue disintegrating following TBS wash on Super Frost Slide?

Hi all, I am using IHC to look at various neuronal markers in zebra finch brain tissue. Finch brains are perfused with PBS and then 4% PFA and placed in a sucrose solution overnight. I then...

01 January 2020 8,400 3 View

Anyone could explain if lentivirus is pathogen?

Because lentiviral vectors are derived from the HIV-1 pathogen, biosafety issues are an important concern. I was asked to indicate whether if lentivirus is animal or human pathogen when I try to...

10 July 2019 2,950 3 View

How to rear aquatic Ceratopogonidae larvae?

11 April 2017 184 13 View

What is the suitable culture condition for culturing cryopreserved PBMCs for 2-3days to obtain maximal viability rate using flow cytometry?

I have been trying to culture cryopreserved PBMCs, but PBMCs with just media alone (after 48 hours) were giving me very low viability rate when acquired on a flow cytometry. The viability rate...

10 May 2015 6,353 18 View

Does anyone have experience with making lentivirus with "Lentivirus Precipitation Solution" from ALSTEM?

We are going to concentrate lentivirus by using this kit, however, I could not find any published reference about it.

18 January 2015 6,150 3 View

Which pipeline or approaches to predict sRNA's in bacteria?

I'm trying to predict some sRNA's (small rna's) which are non-coding rna's in bacteria. I'm following an approach that i have seen in a paper, that uses MAUVE and RNAz to predict them. But the...

07 April 2014 5,769 2 View

Why can't a statistical test of significance (inferential analysis) be applied to a population?

Or can it be?

23 March 2014 120 13 View

Are the quotients of 1/0 and 0/0 the same?

Their quotients in the calculator are both "error". Math books show they are both infinity.

22 March 2014 5,978 67 View

Is there any study available containing the results of the validity test of its data-collection instrument?

Or is there any study that measures the validity of a questionnaire?

09 January 2014 4,871 2 View

Preparation of MacConkey + 1.0mg/L cefotaxime

Hey guys, I need to prepare 400 mL MacConkey + 1.0 mg/L cefotaxime. However, I don't know how to make the stock solution for this antibiotics. Can someone help? Thanks

01 January 1970 3,773 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View