Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Can Microplastics act as vector of chemical transport into human system on the model of stomach oil extraction ?

Chemical transport from micro-plastics to animal tissues has been reported in birds.

01 August 2019 9,216 1 View

I need more article on the assessment of nutritional status in school age children?

01 July 2019 6,210 2 View

How do You determine the ranges of value for your parameters, time and temperature using soxhlet extraction(N-hexane And Ethanol) for lemon grassoil?

I'm trying to conduct an experiment on the optimization of extraction of lemongrass oil using soxhlet extraction. My Optimum parameters are temperature, time and lemongrass to solvent ratio. I'm...

08 April 2019 9,256 1 View

Can I pool sera of chickens within a replicte together for LH FSH ELISA analyses in order to reduce cost of analyses?

I need to run ELISA analyses for reproductive hormones (LH, FSH) in chickens. The samples are however too many. Can I pool sera of birds within a replicate/treatment together for the analyses in...

19 August 2018 9,819 2 View

How do I plot my data using the Sigmoidal Langmuir equation?

I've included an image with showing the equation. I need to know what parameters to plot on the x and y axes. Any help will be highly appreciated.

15 January 2018 290 3 View

Which basis set is the most laudable for studying reactions involving halides?

23 November 2014 5,142 2 View

How to identify the bands from amplification products which appear in gel electrophoresis and how to explain the origin of each of them?

Bands identification in PCR gel electrophoresis

23 May 2014 7,084 2 View

Green organic solvents

I'd like to know if there are any solvents that can be used for the extraction of organic compounds but is less hazardous than say Hexane. Thanks

11 November 2011 5,050 8 View

Is there a way to calculate iron speciation due to pH?

I'd like to be able to figure out what species of iron is predominant at any given pH.

29 September 2011 1,265 22 View

No title

03 March 2021 5,360 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

Has anybody used the Chicken anti-mCherry antibody (ab205402) in immunohistochemistry?

I'm looking for a primary antibody to detect tdTomato in spinal cord slices of mutant mice. I know that the most widely used antibody for this purpose is the mouse anti-RFP from Rockland, but I...

02 February 2021 5,515 3 View

How do I avoid artifacts during paraformaldehyde fixation of mouse CD8+ T cells?

I work with mouse OT-I CD8+ T cells which have been activated in vitro with peptide-MHC and B7 proteins for 3 days. I've been trying to standardize protocols to fix these cells using...

28 January 2021 8,519 2 View

Does re-stimulation of T cells with PMA and Ionomycine before staining for intracellular cytokines for flow cytometry affect reliability of the assay?

I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...

18 January 2021 7,240 7 View