I am trying to extract genomic DNA from frozen PBMCs (already isolated) for use in PCR. Which protocol in the Qiagen QIAamp blood mini kit would be best to use? Any help would be greatly appreciated!
Centrifuge your isolated PBMCs and then discard the supernatant. Add 200 microlitres of lysis solution and 20 microlitres of Proteinase K, briefly vortex, and incubate at 56 deg C for 10 mins, and then follow the kit manufacturer's instructions. Hope it helps.
Centrifuge your isolated PBMCs and then discard the supernatant. Add 200 microlitres of lysis solution and 20 microlitres of Proteinase K, briefly vortex, and incubate at 56 deg C for 10 mins, and then follow the kit manufacturer's instructions. Hope it helps.
We too follow the same protocol as mentioned by @Navonil. We have good yield.
The way blood is processed. Instead of 200ulbof blood, use 200 ul of PBMCs. Test procedure is same.
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