Carmina Coppola

2 Questions 2 Answers 0 Followers

Questions related from Carmina Coppola

What protocol in the QIAamp blood mini kit do you use to extract DNA from isolated PBMCs?

I am trying to extract genomic DNA from frozen PBMCs (already isolated) for use in PCR. Which protocol in the Qiagen QIAamp blood mini kit would be best to use? Any help would be greatly appreciated!

09 October 2020 3,959 4 View

Do these bands in the gel indicate primer-dimers and non-specific DNA product?

I performed qPCR to determine changes in gene expression between undifferentiated C2C12 myoblast cells and differentiated C2C12 cells using KAPA SYBR FAST qPCR Master Mix kit (2x). I prepared four...

08 February 2019 7,384 8 View