Hi Alloysius,

HPLC-UVby using a precolumn derivatization of amino acids could work, if have a purified amino acid samples...

Check this paper out by R. Gatti et. al., (2010)

Article 2,5-Dimethyl-1H-pyrrole-3,4-dicarbaldehyde as a precolumn de...

Hope this helps :)

Dear Jesse,

Thank you so much for answer. The link provided is useful and will help.

We worked with LC MS for the amino acid detection and I prefer LC MS because of its specificity and sensitivity. On the other hand with HPLC we got problem with LOD levels which is not that much great

Dear Karanam Abiram,

Thank you so much for your contribution.

Dear Karanam Abiram,

Thank you so much for your contribution.

Dear Jesse,

I requested for the full paper of the link you provided. Kindly help me with the full paper.

Thank you.

Hi Alloysius, please give your email and I will send the paper to you.