What protocol can I use to analyse amino acid contents of a liquid sample?

We had a product from fermentation of a substrate and desired to know the best approach in analyzing the amino acid content.

10 November 2018 2,295 7 View

How do you calculate colony forming unit per millilitre (CFU/mL) of a bacteria cell using Spectrophotometer?

CFU/mL of sample can easily be determined using solid medium. However, difficulty is always encountered using broth culture in spectrophotometer which may be considered more appropriate by some...

10 November 2018 1,640 12 View

Funding for Conference?

I have need to attend conference in Germany by August and needs funding. Can anyone recommend a funding agency?

05 June 2018 7,300 0 View

How can one estimate the concentration of an aqueous extract of plant material in mg/mL?

The method of estimating the concentration of the extract in mg/mL. Formular and process is required.

01 February 2018 4,242 12 View

Can anyone provide the most suitable approach to study Viable but non-culturable (VBNC) Bacteria?

The VBNC state is a state of dormancy triggered by environmental harsh conditions, such as nutrient starvation, temperature, osmotic stress, oxygen availability, heavy metals, exposure to white...

10 November 2017 7,033 6 View

Can anyone recommend a suitable branded solid medium for the cultivation of methanogenic bacteria from marine environment?

All media available for the enumeration and growth of methanogens seems to be based on formulations to desired composition.

10 November 2017 3,801 6 View

I need a complete protocol to study gene expression in yeasts using real time PCR (q-PCR and RT-PCR) and Microarrays. Can anyone help?

A complete protocol for the study is required

10 November 2017 3,112 7 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View