Chapter The Methanogenic Bacteria

Thank you, Md Iftakharul Muhib for your answer.

A solid medium suitable for the cultivation of methanogenic bacteria (from any environment, also marine) is described in the attached paper. It is a solid basal culture media, the formula is presented at page 520-521. Just be careful to seal the culture media against the oxygen from the air, the simplest way is to add pure paraffin oil on surface.

Carmen Mateescu, thank you so much for your answer and attached file.

Dear Alloysius,

that is quite uneasy task! Many researches mostly evaluated the presence of methanogens by cultivation in liquid medium.

From general point of view I would also suggest to try to use some bacteria-specific antibiotics added to your solid media, if you are going to look for H2-utilizing methanogens. Otherwise you may also get some homoacetogens.

Dear Galushko,

Thank you so much for your answer