Hello, could someone explain to me a procedure for DNA recovery after cyanoacrylate fuming (superglue) + staining. Do you previously decontaminate the surface with bleach or exituplus and then rinse with Miliq water (to remove decontaminating substances)? Which kind of solvent used to dissolve cyanoacrylate layer in the swabbed? Acetone, chloroform...?
Best regards
Hello Rolando,
in our lab this kind of evidence is secured by taking swabbings directly from the item surface. No cleaning, no rinsing etc. before this procedure. If the CA fuming was carried out not long ago (< 1-2 days) you can use pure water or a 1-2% SDS solution; after this period aceton is recommended.
Best regards,
Klaus Berkefeld
Happy New Year. Sorry it has taken so long for me to write and to thank you for your answer.I have had a covid plague in the family. With your advice I will do some tests.
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