Have anyone tried SYBR green with GoTaq DNA Polymerase for a qRT-PCR?

Hi, everyone. I need to perform a real time reverse-transcription PCR in one step. What we have is SYBR green I PCR Master mix with GoTaq DNA Polymerase. So far, our attemps to perform the assay...

07 January 2021 6,507 3 View

Does international survey based researches needs approval in each country ethics commitee?

We plan a contingent valuation of ecosystem services. The study will be based on surveys. We want to apply it in fifteen different countries. Is it necessary to approve the project in ethics...

25 June 2020 8,099 3 View

How to choose the best smooth algorithm for your data in Origin?

I have several curves of applied potential versus electric current with hundreds of points in each of them. I am trying to analyze their first and second derivatives, but they are too noisy to...

27 April 2020 5,932 2 View

I can't make my RAPD-PCR work anymore. Can anyone help me?

Guys, I REALLY NEED HELP At the end of the last year I optimized every parameter of my RAPD-PCR, and as a result I did obtain great products multiple times. Then, I went home for my vacation (I...

18 February 2020 9,236 3 View

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10 December 2019 7,354 5 View

Hi, I would like to solubilize a hidrophobic compound in order to administer it to mices. Should anyone suggest a nontoxic vehicle?

The compound is very insoluble in aqueous solution and the compound administration will be given orally. The compound manufacturer has no information regarding the solubility of the compound....

16 January 2019 834 1 View

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Hello everyone! I have been consulting this webpage http://beast.community/ and http://www.beast2.org/ , but the examples for the use of BEAUti and BEAST are for old versions. The versions of...

02 April 2018 3,942 3 View

Two-step cluster in SPSS - how to validate results?

Hi everybody. After applying a two-step cluster in SPSS, involving both continuous and nominal variables, how can I validate if the results are satisfactory? In other clustering methods there are...

03 October 2017 5,765 8 View

Have you COI sequences of Potamotrygon orbigyi?

24 August 2017 6,634 2 View

How many sequences it needed to put in a crown group to calibrate a tree?

17 August 2017 4,155 2 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

How to find fast publication Scopus Indexed (Q2) journals in Computer Science and Engineering?

Dear Researchers, I would like to find some (Q2) Scopus-Indexed journals with fast publication in the field of Computer Science and Engineering major?

28 February 2021 3,285 2 View

Scopus Index Journal List , 2021?

please provide me the most recent Scopus Index Journals List, February-2021

27 February 2021 3,545 1 View

How can be researcher's index developed?

What are the criteria to be followed to develop the researcher's index?

26 February 2021 9,051 0 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View