Nitrogen fixation is the process of converting atmospheric nitrogen gas (N₂) into ammonia (NH₃), a usable form of nitrogen for plants and other organisms. This seemingly simple process is actually quite complex and energetically demanding, requiring a special enzyme called nitrogenase.
Most nitrogen fixation is performed by bacteria, specifically:
Free-living bacteria: These bacteria, such as Azotobacter and Clostridium, live independently in the soil or water.
Symbiotic bacteria: These bacteria, such as Rhizobium and Frankia, live in a mutually beneficial relationship with plants, typically legumes like beans and peas. In exchange for sugars and other nutrients from the plant, the bacteria provide the plant with fixed nitrogen.
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Nitrogen fixation process diagram
Nitrification
Nitrification is the two-step process of converting ammonia (NH₃) into nitrate (NO₃), another usable form of nitrogen for plants. This process is carried out by chemoautotrophic bacteria, which means they get their energy from the oxidation of inorganic compounds. The two main types of nitrifying bacteria are:
Nitrosomonas: These bacteria convert ammonia to nitrite (NO₂).
Nitrobacter: These bacteria convert nitrite to nitrate.
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Nitrification process diagram
Denitrification
Denitrification is the process of converting nitrate (NO₃) back into atmospheric nitrogen gas (N₂). This process is carried out by anaerobic bacteria, which means they can live without oxygen. Denitrification occurs in environments with low oxygen levels, such as waterlogged soils or the deep ocean.
Here's a table summarizing the types of organisms that perform each process:
These three processes are essential for maintaining the balance of nitrogen in the environment. Nitrogen fixation makes atmospheric nitrogen available to living organisms, nitrification provides plants with a usable form of nitrogen, and denitrification returns nitrogen to the atmosphere, where it can be used again for nitrogen fixation.
Nitrogen fixation takes place in a wide variety of bacteria, the best known of which is rhizobium which is found in nodules on the roots of leguminous plants such as peas, beans, soya and clover. Nitrogen fixation is carried out naturally in soil by microorganisms termed diazotrophs that include bacteria, such as Azotobacter, and archaea. Some nitrogen-fixing bacteria have symbiotic relationships with plant groups, especially legume. Organisms that are involved in nitrogen cycles are nitrogen fixers, nitrifying bacteria, and denitrifying bacteria. As: Nitrosomonas, Rhizobium, Pseudomonas, and Thiobacillus, etc. There, the ammonification, nitrification, and denitrification processes are performed by marine bacteria and archaea. Denitrification by bacteria converts nitrates (NO3−) to nitrogen gas (N2). Nitrification by bacteria converts nitrates (NO3−) to nitrites (NO2−). Nitrogen fixing bacteria convert nitrogen gas (N2) into organic compounds. Denitrifying microorganisms involve a wide range of bacterial groups, such as Bacillus, Enterobacter, Micrococcus, Pseudomonas, Spirillum, Proteus, Aerobacter, and Flavobacterium. The difference between nitrogen fixation and denitrification is that nitrogen fixation is the process in which converts molecular nitrogen to ammonia or other nitrogenous compounds. Denitrification is the opposite in that nitrates are reduced to produce molecular nitrogen.Common nitrifying microorganisms include Nitrosomonas, Nitrosococcus. Nitrobacter, Nitrospira, Nitrosopumilus maritimus, and Nitrososphaera viennensis. Common denitrifying microorganisms include Paracoccus denitrificans, Thiobacillus denitrificans, Proteobacteria.Nitrogen-fixing bacteria are bacteria that convert free atmospheric nitrogen into ammonia or ammonium in soil. Denitrifying bacteria are bacteria that convert nitrates in the soil to free atmospheric nitrogen. So, this is the key difference between nitrogen-fixing bacteria and denitrifying bacteria.