Anti-Src and anti-pY418 Src antibodies for IF?

Hi everyone, Does anyone have a suggestion for anti-Src and anti-pY418 Src antibodies that work well in immunofluorescence on cultured neurons? Thanks a lot!

26 January 2021 866 1 View

What is the impact of philanthropically-minded entrepreneurs in the institutionalization of social innovation?

Curious about the idea, that philanthropically-minded entrepreneurs can help to diffuse/institutionalize social innovation due to their belonging to at least two matured fields of business and...

12 August 2020 4,117 3 View

Are there any tools to extract data from facebook pages (posts, comments, etc.)?

Since it is not possible to work with Netvizz anymore I was hoping to find another tool which works similarly.

26 March 2020 959 3 View

Is there any reason why I can not order an oligo and use it directly as an insert for SLiCE cloning?

I was about to design the primers for SLiCE, when I realized the insert is so small I could order it as one oligo and it would not even be that expansive. As its quite some time ago, that I used...

18 March 2020 2,996 3 View

Sars-cov-2 (Covid-19): qPCR or serological antibody testing - is there information?

So far I only read about PCR testings to confirm a patients infection. Is somebody aware of other diagnostical testing options? E.g. for EBV (Ebstein Barr Virus) exists a serology testing method...

12 March 2020 3,965 6 View

Which problems in fire fighting could be addressed with mobile robots? For which industrial scenarios would such a fire fighting robot be helpful?

I am looking for narrowing down the topic for my master theses. Real industrial problems to be solved with innovative technologies are the most interesting topics.

30 April 2019 5,506 6 View

What are some good examples (case studies) for using open badges in higher education?

I am writing a post about open badges, and researching some examples.

02 April 2019 4,484 2 View

Can anyone suggest a software for designing primers and internal oligos on bisulfite converted DNA, instead of Beacon Designer?

07 June 2018 6,063 2 View

Programs to open .gel files to quantify Western Blot images other than ImageQuant TL?

I don't have access to ImageQuant TL as you need to pay for a license, are there any similar programs that are free for opening .gel files?

19 April 2018 6,565 2 View

Suggestions for reliable online questionnaires to evaluate neuropsychiatric disorder symptoms?

Can anyone recommend validated online (web-based) questionnaires to evaluate neuropsychiatric disorders/symptoms?

09 October 2017 4,684 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Are we using now IT technology effectively?

I discover now two my papers, which are more then 30 years old and which are describing something, what was done in years 1985 -...

21 February 2021 5,008 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View