What PCR protocol do you use to diagnose Porcine Circovirus Type 2?

More Maria Serena Beato's questions See All

Is anyone working with PK15 cell line free form circovirus?

I would like to find PK15 cell line free of Circovirus to attempt PCV2 isolation.

04 May 2015 5,894 2 View

How do you titre your RDE batch for HI test?

We followed a protocol that does not work. :(

02 March 2014 2,001 1 View

Does anyone use the tissueLyser II Qiagen (equipment) to disrupt organs for virus isolation in cell culture?

We stiIl use pestle and mortar which is very time-consuming and we would like to speed up this process.

01 February 2014 9,646 8 View

What kind of ELISA test do you use for serological diagnosis of influenza in swine?

ELISA for H1, H3 and pH1.

05 June 2013 8,775 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Lester J. Perez

Hi, Maria Serena, you can use the rPCR reported by Olvera et al., 2004 ( you can find enclosed a copy) this assay is based on taqman probe principle. You can also use the method published by our group (you can also find enclosed a copy) which is based SYBR-green.

kind regards

Lester

Maria Serena Beato

Great! thank you very much. I will be back to you in case I have some questions.

Maria Serena

Siamak Zohari

Dear Maria Serena,

I hope that you are fine! We use an inhouse taqman qRT-PCR based on 125bp ORF1 replacase of PCV-2 with Pri-pro ET-system. I'm currently outof office but if you are intressted I can send you a detail protocol early nex tweek.

I would like to take the opportunity to wish you and your family a happy and healthy new year. I hope 2015 brings a lot of success in your life and work.

Cheers

Siamak

Hi Siamak

thanks a lot and Happy new year.

yes it would be nice to talk about that.

[email protected]

If you receive a swine lung what ype of PCR do you use?