What might explain the inconsistent results and lower values in the HPLC analysis for the inhibitory effects of fatty acids on CYP3A4 activity?

26 February 2025 0 2K Report

Hello, I am currently studying about the Inhibitory Effects of Saturated and Polyunsaturated Fatty Acids on the Cytochrome P450 3A Activity in Rat Liver Microsomes, using 6 beta testosterone as the standard. The AUC result from HPLC is as shown here, but I am uncertain how to interpret it as the results seem inconsistent. Additionally, from N1, it appears that the values are significantly lower than usual. Could there be any potential reasons for this?

Thank you for your help.

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