What methods should be used for analyzing PCR amplification products?

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Ruaa SH Redeiny

Article Polymerase Chain Reaction

https://worldwide.promega.com/resources/guides/nucleic-acid-analysis/pcr-amplification/

Dominique Liger

Hi there,

First agarose gel will tell you about the size of the product and possibly about the presence of side products in the reaction. Then you may gel purify the product of interest and then sequence it (using the PCR primers) to get sure it is actually the expected product.