What methods should be used for analyzing PCR amplification products?

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Dhifaf Zeki Popular answer

Agarose gel electrophoresis

Ruaa SH Redeiny

Article Polymerase Chain Reaction

https://worldwide.promega.com/resources/guides/nucleic-acid-analysis/pcr-amplification/

Abhijeet Singh

Damya Kareem

What do you mean by analysis? What do you want to do?

Dominique Liger

Hi there,

First agarose gel will tell you about the size of the product and possibly about the presence of side products in the reaction. Then you may gel purify the product of interest and then sequence it (using the PCR primers) to get sure it is actually the expected product.

Rasha Shakir Nima

Determine the pcr product weight by electrophoresis

Dhifaf Zeki

Chapter Analysis of PCR Amplification Products

Naser Jawad Kadhum