What methods can you recommend for separating trans and cis isomers of isoquinolinone, given my unsuccessful attempts with various solvent ratios?

26 September 2024 1 1K Report

I am synthesizing an organic compound (isoquinolinone) that has both trans and cis isomers, which are very similar in structure. Despite trying various solvent ratios for separation, I have not been successful. What alternative methods or techniques could you suggest for effectively separating these isomers?

E.A. Gawad

you can deploy Two Minute Separation of the cis- and trans-Isomers of Vitamin K1 without Heptane, Chlorinated Solvents, or Acetonitrile Article Two Minute Separation of the cis- and trans-Isomers of Vitam...

also some advices from AI Here are a few alternative methods you might consider for separating trans and cis isomers of isoquinolinone:

Chiral Chromatography: This technique uses a chiral stationary phase to separate enantiomers and geometric isomers based on their interactions with the chiral environment12. It's particularly useful for compounds with subtle structural differences.

Supercritical Fluid Chromatography (SFC): SFC uses supercritical CO₂ as the mobile phase, which can offer better resolution for isomers compared to traditional liquid chromatography3. It's also environmentally friendly and efficient.

High-Performance Liquid Chromatography (HPLC): Using a specialized column, such as a C18 or silica column, with an appropriate gradient of solvents might help achieve better separation2.

Gas Chromatography (GC): If the isomers are volatile, GC can be an effective method. You can use a non-polar or slightly polar stationary phase to achieve separation.

Crystallization: Sometimes, crystallizing the mixture under different conditions (temperature, solvent, etc.) can lead to the selective crystallization of one isomer over the other.

Preparative TLC (Thin-Layer Chromatography): This method can be used for small-scale separations. By optimizing the solvent system, you might achieve better separation.

Recrystallization: If the isomers have different solubilities in various solvents, recrystallization can be a useful method to separate them.

https://www.chromforum.org/viewtopic.php?t=24310

Separation of Isomers of Vitamin K1 Using Normal Phase HPLC

Separation of Isomers of Vitamin K1 Using Normal Phase HPLC (thermofisher.com)

Best wishes

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