Is my first time working with stem cells and I don't what kind of stem cells are better for this.
Differentiating Neural Stem Cells into Neurons and Glial Cells
https://www.thermofisher.com/hk/en/home/references/protocols/neurobiology/neurobiology-protocols/differentiating-neural-stem-cells-into-neurons-and-glial-cells.html
The easiest thing is to add 0.5-1% FCS into the media, lowering the concentrations of EGF and FGF. You get differentiation in 24-48 hr with he cells fully differentiated in 72 hours.
It would be helpful if you specify which kind of neurones do you want to obtain. There are plenty of protocols published and some commercial kits that can be useful (I would recommend you to better find a protocol than a commercial kit since they are quite expensive).
And also what kind of stem cells: if you want to start with pluripotent stem cells (embryonic or iPS) or if you're starting directly with neural stem cells, if they are human or from any other source, etc. Then I guess people might give you more specific answer.
@PedroMiguelBelioMairal: I am considering both options, but probably I will start with pluripotent stem cells. And I trying to write my own protocol based on other projects, but at the end of the day, I just looking for the best and cheaper option. I really appreciate your suggestions.
@JoseAGarcia-Sanz: I want to know for what kind of stem cells this will work and what kind of neurons I can get using FCS.
If you're looking for the cheapest option, I would go for the protocol, better than commercial kits. And you also have the advantage that some published protocols achieve to have certain types of specific neuronal types, while commercial kits usually give mixed neuronal populations. I would recommend you first to try a published protocol, if you have no experience with stem cells, trying to establish your own protocol of differentiation can be a huge nightmare. Once you already know how the protocol works, you can try to improve it by your own ideas.
If you're looking for specific disease, I would recommend you to start with pluripotent stem cells (better if you choose iPS cells, easier to find a specific disease cell line, check here for example: https://catalog.coriell.org/ ). This, if you want human cells, of course; if you are going to work with mice, you can use embryonic stem cells. Another advantage with PSC is that you can differentiate your own neural stem cells (since you have PSC, you have an "unlimited" source of neural stem cells) and freeze them for further experiments.
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