What methods are best for quantifying glucose and xylose in the mixture of the two in a broth culture? I am doing a research on bioethanol from xylose but HPLC is not within my reach.
Based on my experience-TLC method is best especially for xylose and glucose.
Dear Nweze,
The best way is to use enzymatic measurements.
We do use the dosing kits developed by the French society LIBIOS.
Here is their website:
http://www.libios.fr/index.php?&_lang=GB
I also enclosed the technical files of the tow dosing kits you'll need: one is dosing glucose and the second specifically xylose.
Do not hesitate to contact them, they are very friendly.
Best regards and good luck for your experiments.
Prof Philippe Urban
Thanks for the information.
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