Dear Nweze,

          Which could not be determined experimentally rather you can refer the following article.

               Acta Chim. Slov.2005,52,435–439

You can do perform reducing sugar assay using DNS method. this will give you total reducing sugars in the broth. and simultaneously perform GOD-POD assay which is specific for glucose. Alternatively you can estimate xylose using Bial's test (using orcinol reagent) which is specific for pentose sugars,

Dear Reasearcher,

I suggest you to use orcinil method for xylose and GOD=POD for glucose estimations.

Best Regards,

Sivamani S

you may use HPLC

agreed what Dr Deepti indicated

Ysi glucose analyzer can be used for glucose and DNS method can be used for the reducing sugars avaliable in the broth.

The best method for quantifying glucose and xylose in the mixture is HPLC, but when they are not available the DNS method used for determination total reducing sugar.

Best method is to use a YSI biocehmeistry analyser with both glucose and xylose chemistry...it is highly sensitive as it is biosensor based

I know HPLC is the best but it is currently not available. Thanks for your contributions.

DNS

The total reducing sugars produced from the enzymatic hydrolysis

ofcellulose can be measured by the dimtrosalicylic acid (DNS)

method (Miller, 1959; Andreotti, 1980), as follows:

1. 3,5-dimtrosalicylic acid

2. NaOH

3. distilled water

4. Rochelle salts (sodium potassium tartrate)

5. phenol (melt at 50°C)

6. sodium metabisulfite

7. phenolphthalein solution

8. 0.1NHCL

9. 1 g/ell )glucose (Dextrose) standard solution

10. spectrophotometer

1. Dissolve the 10.6 g 3,5-dinitrosalicylic acid and 19.8 g NaOH

into 1416 mL distilled water in a stirred beaker, then add 306 g

Rochelle salts, 7.6 mL (8.132 g) phenol, and 8.3 g sodium

metabisulfite.

2. Titrate 3 mL sample with phenolphthalein with 0.1 N HC1. It

should take 5-6 mL of 0.1 N HC1. Add NaOH into the preceding

DNS solution if required (2 g NaOH for the additional 1 mL 0.1

N HCl).

Assay Procedures:

1. Dilute the standard glucose solution to make 0.2, 0.4, 0.6, 0.8,

and 1.0 g/ell solutions. Use these known gilicose solutions as

samples for step and Prepare a calibration curve which

shows the absorbance versus glucose concentration.

2. Dilute a sample so that the expected concentration is in the

range of the calibration curve.

3. Place 1 mL sample in a test tube and add 3 nl L ONS reagent.

Place in boiling water for 5 minutes. Cool to room temperature.

4. Read light absorbance at 550 nm with a water blank.

5. Read the value of glucose concentration corresponding to the

absorbance from the calibration curve.

Thank you all.