We will use protein as one of the contaminants on lab glassware
Use BSA, it is relatively inexpensive and everyone is using it for protein assay. That way, your study can be readily replicated elsewhere.
My colleagues and I are currently conducting a survey with Likert-scale items and a portion of the survey allows the respondents to add answers which were not included in the prior items (e.g....
30 May 2022 4,816 2 View
I want to simulate satellite attitude control with a quaternion attitude representation. thrusters and reaction wheels are the main AOCS controllers. is it Ok to omit orbit dynamics (orbit...
31 January 2021 5,393 2 View
I have stimulated macrophages to become pro-inflammatory (M1) and anti-inflammatory (M2) and I have unstimulated control group that was treated with with vehicle, PBS (M0). I would like to compare...
16 August 2020 9,838 4 View
I have done analysis of samples using nanoindenter and now I have to relate this analysis with thermal stresses. Can anyone suggest me how can I see the thermal effects on the samples?
18 November 2019 2,175 0 View
On the day of the experiment, a 1 x 10^7 colony forming units (CFU) in 100 μl of the bacterial suspension was administrated by intraperitoneal injection (i.p.). Your overnight culture of...
23 February 2018 3,964 1 View
I am investigating the puromycin-labelled protein in MSCs. I have taken the integrated density of the immunofluorescence results and Spot blot results. However, what scale do I put for the...
02 October 2017 6,573 1 View
My project is comparing Serum culturing for 30 and 60 minutes and serum free culturing for 30 and 60 minutes. Therefore I am comparing all 4 variable with 5 ratios for each treatment. What would...
21 September 2017 3,777 9 View
I would like to use and/or adapt a survey/questionnaire used in one study of a particular author. The research paper's full text is published in the institution's repositories and is labeled...
05 July 2017 4,637 13 View
I have extracted microbial DNA from Acropora Milliopora, I have to grind all the part of the coral, and I want to separate the calcium carbonate skeleton from slurry of mucus, tissue and skeleton.
10 June 2014 8,952 0 View
I was concentrating my recombinant protein with the amicon spin filters, it was completely dissolved in PBS to begin with, that was around 8mls, but I wanted it concentrated to 10 mg/ml for...
29 May 2014 3,107 2 View
How can parents ensure their drinking water is free from harmful contaminants?
27 July 2024 8,722 6 View
There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...
11 July 2024 4,829 0 View
To combat environmental contaminants, plant tissue culture labs are fumigated. Would vapourised hydrogen peoxide have a negative effect on the tissue cultured plants in jars?
10 July 2024 8,815 1 View
Hi everyone, I'm performing a sparse Partial Least Squares (sPLS) model to understand if the analysed contaminants (55 individual contaminants) explain my response variables (42 response...
07 July 2024 2,333 0 View
Hello everyone. I am culturing hIPSC in mTeSR-1 medium. I found some small round white particles persistently attached to cell colonies, and some actively moved under the 40X objective. Also,...
08 June 2024 6,797 5 View
I am searching for a technical reason that anyone could suggest please?
08 May 2024 6,032 2 View
Hello. I am new to cell culture work. May I know whether these small black dots are contaminants or just cell debris? It's quite confusing because they are not moving. This is different from the...
02 May 2024 8,479 1 View
How can artificial neural networks be applied to optimize key parameters such as adsorbent selection, bed design, and operating conditions in fixed-bed adsorption systems for the efficient removal...
25 April 2024 7,693 0 View
This question is related to the review article titled "Tenets of Specimen Management in Diagnostic Microbiology" by Rajeshwar Reddy Kasarla and Laxmi Pathak. The article states that optimal...
11 April 2024 4,550 1 View
To quote a report from King County, WA (Monitoring Stormwater Retrofits in the Echo Lake Drainage Basin - SAM Effectiveness Study), "At each BMP site, effluent concentrations for most contaminants...
02 April 2024 5,199 3 View