I want to deliver antiviral plasmid into shrimp via feed. Is that possible to integrate the plasmid into animal feed without degrade all of plasmid. What methods that I must use?
Dear Danang,
the important point is to use a vehicle that will highly ciompact and protect the plasmid DNA.
I'm thinking of two options:
the first one is to use a liipd-based that highly compact nucleic acid (such as DreamFect Gold)
the other one is to use magnetic nanoparticles: this was demonstrated some times ago by delivering DNA into jejunum with PolyMag beads (Plank C., Expert Opin Biol Ther. 2003) and also by protecting AdenoVirus into stomach using ViroMag Beads (Scherer F., Gene Ther. 2002; 9(2):102-9).
The important point of the later method is that organims can be immobilized onto a magnetic field in order to enahnce transfection.
If you need any data or information do not hesitate to contact me:
good luck with your experiments,
best regards,
Cedric
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